Article number |
KM4000 |
Tests |
96 Tests |
Volume |
200 µl |
Matrix |
Serum |
Method |
LC-MS/MS, Mode: MRM, Polarity: ESI positive |
The described LC-MS/MS application is intended for the quantitative determination of hepcidin in serum. For in vitro diagnostic use only.
Hepcidin is a small cystein-rich peptide produced in the liver. It regulates the absorp- tion of iron in the body. Hepcidin was initially isolated as a circulating, antimicrobial 25 amino acid peptide in human urine and blood. Human hepcidin is synthesized as prohepcidin, a 84 amino acid precursor, including a putative signal peptide of 24 amino acids. From human urine, two predominant forms, hepcidin-20 and hepci- din-25, di ering by amino-terminal truncation, were characterized as 20 and 25 ami- no acid residues with 8 cysteines connected by intramolecular disul de bonds. An over-expression of hepcidin was shown to result in severe iron de ciency anemia in transgenic mice, indicating that hepcidin plays a pivotal role in iron metabolism. Recent studies have found abnormal hepcidin expression and disrupted hepcidin re- gulation in hemochromatosis gene. A disturbed hepcidin regulation mechanism has a direct e ect on the iron metabolism in the organism. Decreased hepcidin expres- sion, e. g. due to a genetic defect, results in a direct iron overload, known as the iron disease hemochromatosis. Based on these observations, it has been suggested that hepcidin is a key component of iron homeostasis that acts as a negative regulator of iron absorption in the small intestine and of iron release from macrophages.
Hepcidin can be used independently of accompanying diseases as an early predictive marker for functional iron deciency and/or anomalous iron utilization.
For the determination of Hepcidin 25 samples, we developed two sample extraction methods depending on the LC-MS/MS system used.
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Suitable for all LC-MS/MS systems is the procedure using a SPE μElution 96 well plate.
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Suitable for high sensitive LC-MS/MS systems is also a SPE procedure using 1 ml cartridges.
Cat. No. |
Label |
Kit components |
Quantity |
KM4000LA |
MOPHA A |
Mobile phase A |
1000 ml |
KM4000LB |
MOPHA B |
Mobile phase B |
1000 ml |
KM4000KA |
CAL 1 |
Calibrators 1 and 2 (-20°C; lyophilized; concentration, see product specification) |
3 vials each |
KM4000KO |
CTRL 1 |
Controls 1 and 2 (-20°C; lyophilized; concentration, see product specification) |
3 vials each |
KM4000IS |
INT STD |
Internal Standard (lyophilized) |
5 x 2 ml |
KM4000AC |
ACTSOL |
Activation solution |
2.5 ml |
KM4000W1 |
WASH 1 |
Wash solution 1 |
50 ml |
KM4000W2 |
WASH 2 |
Wash solution 2 |
20 ml |
KM4000EL |
ELUSOL |
Elution solution |
10 ml |
KM4000RE |
RECSOL |
Reconstitution solution |
25 ml |
The UPLC separation column (KM4000RP), the pure substances for instrument tuning, as well the μElution plates (KM4000PL) and the Oasis®HLB, 1cc (10 mg) cartridges (KM4000CK) can be ordered separately. The complete Kit as well all individual components can be ordered separately. Please ask for our single component price list.
Application
USA: For Research Use Only. Not for Use in Diagnostic Procedures.